Addition of diadenosine pentaphosphate to creatine kinase reagent improves identification of creatine kinase isoenzymes after electrophoresis.
نویسندگان
چکیده
منابع مشابه
An interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
متن کاملIsoenzymes of creatine kinase; separation by acrylamide gel electrophoresis.
A method for the separation of creatine kinase isoenzymes is described utilizing vertical acrylamide slab electrophoresis. This technique combines the advantages of vertical separation methods with the greater resolving power of acrylamide. The inclusion of the coupling enzymes (hexokinase and glucose-6-phosphate dehydrogenase) in the buffer system should theoretically provide optimal concentra...
متن کاملNOVEL REAGENTS FOR CREATINE KINASE
The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
متن کاملImproved electrophoretic separation of creatine kinase isoenzymes.
Creatine kinase isoenzymes are separated electrophoretically on cellulose acetate, with use of an improved procedure for reactivation and visualization of enzymic activity. N-Acetyl cysteine is used as the reactivator and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide for visualization in a tetrazolium-coupled system. This test, both practical and reliable, is a suitable alter...
متن کاملResults of a nationwide survey of analyses for creatine kinase and creatine kinase isoenzymes.
More than 300 laboratories participated in an interlaboratory survey of creatine kinase (CK, EC 2.7.3.2) determinations in which they analyzed seven lyophilized samples for total CK and CK isoenzymes and furnished information about their methodology. The samples were not necessarily intended to mimic typical patients' specimens but rather to determine the analytical ability of the laboratories ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1983
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/29.3.580a